Optimal Elimination of Leukemic T Cells From Human Bone Marrow With TiOl-Ricin A-Chain Immunotoxin
نویسندگان
چکیده
In view of bone marrow purging before autologous transplantation in T cell malignancies, an anti-human I cell immunotoxin (IT) has been prepared by coupling ricin A-chain to the monoclonal antibody TiOl that binds the Ti differentiation antigen expressed by T lymphocytes as well as by T cell-derived hematologic malignancies. Using a sensitive and reliable clonogenic assay, optimal conditions were defined for the elimination of clonogenic human I Ieukemic cells among bone marrow cells. Maximal cytoreduction was obtained with IT at a dose of 2 tg/mL in the
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